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- W2354865751 abstract "Objective A nested reverse transcription polymerase chain reaction(RT PCR) was developed to study the relationship between expression of AFP mRNA in peripheral blood mononuclear cells and metastasis in patients with hepatocellular carcinoma(HCC).Methods Heparinized blood samples were obtained from 48 patients with HCC,21 patients with chronic liver diseases and 20 normal subjects.Mononuclear cells were separated from blood.Total RNA was extracted by the acid guanidium phenol chloroform method.The first strand of cDNA was synthesized from an aliquot of the RNA using Moloney murine leukemia virus reverse transcriptase and random hexamers.The two pairs of primers chosen from the sequences of AFP were used for the two step PCR.Results AFP mRNA in peripheral blood cells was not detected in all normal subjects, but it was detecte in 28 of 48 (58.33%) cases with HCC and in all 8 patients with extrahepatic metastasis.The positive rate in patients with HCC was significantly higher than the cases with chronic liver diseases(3/21,14.28%, P 0.01).Conclusions The detection of AFP mRNA in peripheral blood cells may be helpful for clinial detection of metastasis of HCC.$$$$" @default.
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- W2354865751 date "1999-01-01" @default.
- W2354865751 modified "2023-09-25" @default.
- W2354865751 title "EXPRESSION OF AFP mRNA IN PERIPHERAL BLOOD CELLS OF PATIENTS WITH HEPATOCELLULAR CARCINOMA" @default.
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