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- W2354899684 abstract "Objective To observe the distribution of small interference RNA(siRNA)7816 in mice and the efficacy of siRNA-based therapy on respiratory syncytial virus(RSV)infection. Methods The siRNA labeled with cy3 fluorophore were intranasally introduced into the lung tissue of the mice.The mice were killed at 12 hours after infection,and their lung,liver and intestines were removed for immediate frozen section.The distribution of fluorescein-labeled siRNA was then observed under the fluorescence microscope.Forty-eight Balb/c mice aged 8-12 weeks were randomly divided into 6 groups(n=8).The mice was given 1010 pfu·L-1 standard RSVA2 intranasally.On the 2nd day after RSV infection,the 3 treatment groups were given 0.5 nmol,1.0 nmol and 2.0 nmol siRNA respectively,the negative control group was gi-ven 1.0 nmol negative siRNA,the phosphate buffered saline(PBS) group was given PBS,and the blank control group was given nothing.The mice were killed on the 3rd day post-infection,and their lung was removed under sterile condition.The efficacy of siRNA7816 inhibiting RSV was analyzed through pathological examination of the lung under light microscope and measurement of the optic density of the immunohistochemical slide image. Results High concentration of siRNA7816 was detected in bronchi and pulmonary alveoli following intranasally administration of the siRNA to the mice.Pathological examination of the lung tissue showed that siRNA significantly alleviated the inflammation.Immunohistochemistry and optical density detection indicated that siRNA7816 inhibited the replication of RSV(P0.05). Conclusion siRNA7816,following intranasally administration,successfully reaches the lung tissue of the mice and inhibites the replication of RSV." @default.
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- W2354899684 date "2011-01-01" @default.
- W2354899684 modified "2023-09-27" @default.
- W2354899684 title "Distribution of Small Interference RNA and Efficacy of Small Interference RNA-Based Therapy on Respiratory Syncytial Virus Infection in Mice" @default.
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