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- W2354915084 abstract "Objective:To establish a RP-HPLC method for simultaneous determination of the contents of chloromycetin and berberine hydrochloride in chloromycetin and berberine ointment.Methods:The separation was performed on a Kromasil C18 analytical column (150 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile-0.033 mol/L potassium dihydrogen phosphate (30:70).The detection wavelength was 265 nm,the column temperature was room temperature,the flow rate was 1.0 ml/min and the sample injection volume was 20 μl.Results:The standard curves were linear in the range of 0.82-26.25 μg/ml for chloromycetin (r=0.999 9) and 0.79-25.35 μg/ml for berberine hydrochloride (r=0.999 9).The average recovery rates of chloromycetin and berberine hydrochloride were (96.53±0.89)%(n=3) and (99.58±3.50)%(n=3),respectively.The average contents of chloromycetin and berberine hydrochloride in three batch samples were (95.75±0.52)% and (98.54±0.66)% of the labeled amount,respectively.Conclusion:The method is simple and accurate,and can be used for quality control of chloromycetin and berberine ointment." @default.
- W2354915084 created "2016-06-24" @default.
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- W2354915084 date "2010-01-01" @default.
- W2354915084 modified "2023-09-24" @default.
- W2354915084 title "Simultaneous determination of the contents of chloromycetin and berberine hydrochloride in chloromycetin and berberine ointment by RP-HPLC method" @default.
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