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- W2354915221 abstract "The derivative of olaquindox,called as OLA-SH,was synthesized by succinic anhydride method and identified by mass spectrometry(MS).Two complete antigens of olaquindox(OLA-BSA,OLA-OVA) were prepared by active ester method(AE).The characterization of hapten-protein conjugates were examined by two methods,ultraviolet spectrophotometry(UV),and sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE),respectively.BALB/C mice were immunized with OLA-BSA,the titre of polyclonal antibody(pAb)was detected by indirect ELISA,the sensitivity and specificity of pAb was identified by blocking ELISA.According to UV scanning,the conjugation ratio of OLA-HS to BSA and OVA was about 17.1∶1 and 12.4∶1.The titer of pAb for No.3 mouse was the highest,its IC50 was 58.923 ng/mL,cross reaction rate to carbadox was 1.841%,and no cross reaction to other antibiotics.The high-titer,sensitive and specific anti-OLA pAb have been produced in sera of ELISA,which made it possible to establish immunoassay of OLA residues." @default.
- W2354915221 created "2016-06-24" @default.
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- W2354915221 date "2010-01-01" @default.
- W2354915221 modified "2023-09-25" @default.
- W2354915221 title "Synthesis and Characterization of Artifical Antigen of Olaquindox" @default.
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