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- W2354951570 abstract "Objective:To compare the effect of slow-freezing,vitrification using two different carriers (cryoloop and self-made blade) on cryopreservation of human oocytes.Methods:After obtaining patients'consent and with approval of the Hospital Ethics Committee,we collected immature MI oocytes,some of them were cryopreserved after naturally in vitro mature.121 oocytes were thawed,in which surviving oocytes were fertilized by ICSI.Compare the difference on survival rate,fertilization rate,cleavage rate,good embryo rate between slow-freezing and vitrification (cryoloop and self-made blade).Meanwhile,the corresponding parameters of normal fresh MⅡoocytes after ICSI was compared with thawed oocytes.Results:Vitrification of human oocytes can obtain better survival rate,significantly higher than slow-freezing; vitrification-thaw oocytes showed no difference on fertilization rate with normal fresh MⅡoocytes after ICSI,but their cleavage rate and good embryo rate were significantly lower than normal fresh MⅡ oocytes.Conclusion:Vitrification of human oocytes can obtain better survival rate;cryoloop and self-made blade are ideal vitrification carriers,these two carriers have close effect,and self-made blade is lower cost and easy to use;the post-thaw developmental potential of naturally in vitro maturated oocytes reduces after vitrification.Cryopreservation of human oocytes needs more studies." @default.
- W2354951570 created "2016-06-24" @default.
- W2354951570 creator A5024696000 @default.
- W2354951570 date "2010-01-01" @default.
- W2354951570 modified "2023-09-23" @default.
- W2354951570 title "A pilot study on cryopreservation of human oocytes" @default.
- W2354951570 hasPublicationYear "2010" @default.
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