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- W2355006431 abstract "The expression vector containing human interleukin-1 receptor antagonist(HuIL1ra) gene was transformed into Escherichia coli BL21(DE3).The transformant produced recombinant HuIL-1ra as an inactive inclusion body.Protein purification was achieved by solubilization,on-column refolding,and single anionic exchange chromatography in the presence of 1 mmol/L dithiothreitol,successively.The as-purified protein was in its reduced form,which easily shifted to the corresponding oxidized form when exposed to air,glutathione,or Cu~(2+).The two forms were separated by reverse phase high performance liquid chromatography;however, non-reducing sodium dodecyl sulfate polyactrylamide gel electrophoresis data suggested near identical molecular weight,implying an intramolecular disulfide linkage in the oxidized form.The oxidized form of recombinant HuIL-1ra showed better stability with good biological activity." @default.
- W2355006431 created "2016-06-24" @default.
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- W2355006431 date "2005-01-01" @default.
- W2355006431 modified "2023-09-25" @default.
- W2355006431 title "Purification of recombinant human interleukin-1 receptor antagonist and analysis of its two different oxidative forms" @default.
- W2355006431 hasPublicationYear "2005" @default.
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