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- W2355117811 abstract "OBJECTIVE:To investigate the expression of glucosylceramide synthase(GCS) gene in human MCF-7 cells and MCF-7/ADR (doxorubicin-resistant) breast cancer cells and its significance. METHODS: MTT was used to detect the IC50 of MCF-7 and MCF-7/ADR to doxorubicin; exposing MCF-7/ADR to various concentrations of PDMP for different time, then the IC50 of MCF-7/ADR was detected. FCM was employed to determine the expression of GCS protein in MCF-7 and MCF-7/ADR before and afer pretreatment with PDMP; real time RT-PCR was adopted to evaluate the expression of GCSN in them. RESULTS: The IC50 of MCF-7/ADR and MCF-7 were (18.95±0.54) and (0.84±0.07) μg·mL-1; the drug resistance of MCF-7/ADR was 22.69 times that of MCF-7. PDMP exposure enhanced doxorubicin sensitivity, it decreased the IC50 of MCF-7/ADR to (5.63±0.58) μg·mL-1. The expressions of both GCS and GCSN in MCF-7/ADR were significantly increased compared to MCF-7 cells by FCM and real time RT-PCR (P0.01). PDMP exposure decreased of the expression of GCSN from 0.004 8±0.001 7 to 0.002 1±0.000 4. CONCLUSIONS: GCS might be involved in the development of drug resistance in malignant carcinoma and play an important role in the drug resistance of MCF-7/ADR." @default.
- W2355117811 created "2016-06-24" @default.
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- W2355117811 date "2008-01-01" @default.
- W2355117811 modified "2023-09-28" @default.
- W2355117811 title "Expression of Glucosylceramide Synthase in Human Doxorubicin-resistant Breast Cancer Cells and Its Significance" @default.
- W2355117811 hasPublicationYear "2008" @default.
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