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• W2355131055 abstract "The 3T3-L1 cell line is one of the most well-characterized and reliable models for studying adipocytes. Increased oxidative stress in accumulated fat was found in 3T3-L1 cells. Berberine, an isoquinoline alkaloid, could suppress fat deposition in 3T3-L1 cells; however, whether berberine suppresses increased oxidative stress is not well known. In this study, we observed the effect of berberine on increased oxidative stress in 3T3-L1 cells. 3T3-L1 cells were cultured and treated with berberine (5–20μM) from day 3 to day 8. We confirmed that berberine markedly inhibited fat accumulation and lipid droplets in 3T3-L1 adipocytes and decreased triglyceride content. Berberine inhibited increased oxidative stress in 3T3-L1 cells by suppressing reactive oxygen species (ROS) production, and increased glutathione peroxidase (GPx) gene expression and GPx activity. Berberine also markedly reduced adipokines secreted by adipocytes, including leptin and resistin." @default.
• W2355131055 created "2016-06-24" @default.
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• W2355131055 date "2015-06-01" @default.
• W2355131055 modified "2023-09-26" @default.
• W2355131055 title "Increased Oxidative Stress in Cultured 3T3-L1 Cells was Attenuated by Berberine Treatment" @default.
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• W2355131055 doi "https://doi.org/10.1177/1934578x1501000626" @default.
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• W2355131055 hasPublicationYear "2015" @default.
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