Matches in SemOpenAlex for { <https://semopenalex.org/work/W2355143312> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2355143312 endingPage "70" @default.
- W2355143312 startingPage "366" @default.
- W2355143312 abstract "To observe the effect of integrin beta8 on the neuronal apoptosis after hypoxia ischemia (HI) in astrocyte/neuron co-culture system.Astrocytes and neurons were cultured in vitro from cerebral cortex of the P1-3 days Sprague Dawley rats and E16 days fetal rats, respectively. Immunocytochemistry staining was used to identify the purity of cells. Integrin beta8 mRNA expression was qualified in the astrocytes at 12 hours, 1 day, and 2 days after HI and reoxygenation (experimental group) and in normal astrocytes (control group) by RT-PCR. Integrin beta8 small interering RNA (siRNA) system was established to specifically block astrocyte beta8 expression, the efficiency of integrin beta8 inhibition was detected by real-time fluorescent PCR. The astrocytes and neurons were co-cultured to established the astrocyte/neuron co-culture system. The neuronal apoptosis was detected with TUNEL in the normal neurons/astrocytes group (co-cultured HI group), the astrocytes infected by integrin beta8 siRNA for 2 days/normal neurons group (beta8 RNA interference group), and normal neurons in vitro with HI treatment group (HI group) at 1 day after HI and reoxygenation. The normal neurons without treatment as control (control group).Glial fibrillary acidic protein and neuronal nuclei staining suggested a purity of more than 90% in cultured cells. HI resulted in an increase of integrin beta8 mRNA expression at 12 hours after reoxygenation in astrocytes, which peaked at 1 day after reoxygenation, then slowly decreased and remained higher at 2 days, showing significant differences between control group and experimental group and among different time points in experimental group (P < 0.05). RNA interference efficiency was most significant at 2 days after astrocytes infected with integrin beta8 siRNA (P < 0.05). The neuronal apoptosis was significantly increased in HI group, co-cultured HI group, and beta8 RNA interference group when compared with control group (P < 0.05). But neuronal apoptosis index (AI) was significantly decreased in co-cultured HI group and beta8 RNA interference group when compared with HI group (P < 0.05). The significant difference of AI was found between co-cultured HI group and beta8 RNA interference group (P < 0.05).Integrin beta8 expression can be induced with hypoxic-ischemic brain damage, leading to decreased AI of neurons and obvious protective effect." @default.
- W2355143312 created "2016-06-24" @default.
- W2355143312 creator A5002683016 @default.
- W2355143312 creator A5004861529 @default.
- W2355143312 creator A5023789866 @default.
- W2355143312 creator A5024080874 @default.
- W2355143312 creator A5056073315 @default.
- W2355143312 creator A5060718181 @default.
- W2355143312 date "2014-03-01" @default.
- W2355143312 modified "2023-10-16" @default.
- W2355143312 title "[Effect of integrin beta8 on neuronal apoptosis after hypoxia ischemia in astrocyte/neuron co-culture system]." @default.
- W2355143312 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24844022" @default.
- W2355143312 hasPublicationYear "2014" @default.
- W2355143312 type Work @default.
- W2355143312 sameAs 2355143312 @default.
- W2355143312 citedByCount "1" @default.
- W2355143312 countsByYear W23551433122016 @default.
- W2355143312 crossrefType "journal-article" @default.
- W2355143312 hasAuthorship W2355143312A5002683016 @default.
- W2355143312 hasAuthorship W2355143312A5004861529 @default.
- W2355143312 hasAuthorship W2355143312A5023789866 @default.
- W2355143312 hasAuthorship W2355143312A5024080874 @default.
- W2355143312 hasAuthorship W2355143312A5056073315 @default.
- W2355143312 hasAuthorship W2355143312A5060718181 @default.
- W2355143312 hasConcept C126322002 @default.
- W2355143312 hasConcept C134018914 @default.
- W2355143312 hasConcept C153911025 @default.
- W2355143312 hasConcept C16685009 @default.
- W2355143312 hasConcept C169760540 @default.
- W2355143312 hasConcept C170493617 @default.
- W2355143312 hasConcept C178790620 @default.
- W2355143312 hasConcept C185592680 @default.
- W2355143312 hasConcept C190283241 @default.
- W2355143312 hasConcept C195687474 @default.
- W2355143312 hasConcept C196795494 @default.
- W2355143312 hasConcept C203014093 @default.
- W2355143312 hasConcept C204232928 @default.
- W2355143312 hasConcept C2777542381 @default.
- W2355143312 hasConcept C2778794669 @default.
- W2355143312 hasConcept C2780495277 @default.
- W2355143312 hasConcept C2780884517 @default.
- W2355143312 hasConcept C35866371 @default.
- W2355143312 hasConcept C529278444 @default.
- W2355143312 hasConcept C540031477 @default.
- W2355143312 hasConcept C541997718 @default.
- W2355143312 hasConcept C55493867 @default.
- W2355143312 hasConcept C71924100 @default.
- W2355143312 hasConcept C7836513 @default.
- W2355143312 hasConcept C86803240 @default.
- W2355143312 hasConcept C95444343 @default.
- W2355143312 hasConceptScore W2355143312C126322002 @default.
- W2355143312 hasConceptScore W2355143312C134018914 @default.
- W2355143312 hasConceptScore W2355143312C153911025 @default.
- W2355143312 hasConceptScore W2355143312C16685009 @default.
- W2355143312 hasConceptScore W2355143312C169760540 @default.
- W2355143312 hasConceptScore W2355143312C170493617 @default.
- W2355143312 hasConceptScore W2355143312C178790620 @default.
- W2355143312 hasConceptScore W2355143312C185592680 @default.
- W2355143312 hasConceptScore W2355143312C190283241 @default.
- W2355143312 hasConceptScore W2355143312C195687474 @default.
- W2355143312 hasConceptScore W2355143312C196795494 @default.
- W2355143312 hasConceptScore W2355143312C203014093 @default.
- W2355143312 hasConceptScore W2355143312C204232928 @default.
- W2355143312 hasConceptScore W2355143312C2777542381 @default.
- W2355143312 hasConceptScore W2355143312C2778794669 @default.
- W2355143312 hasConceptScore W2355143312C2780495277 @default.
- W2355143312 hasConceptScore W2355143312C2780884517 @default.
- W2355143312 hasConceptScore W2355143312C35866371 @default.
- W2355143312 hasConceptScore W2355143312C529278444 @default.
- W2355143312 hasConceptScore W2355143312C540031477 @default.
- W2355143312 hasConceptScore W2355143312C541997718 @default.
- W2355143312 hasConceptScore W2355143312C55493867 @default.
- W2355143312 hasConceptScore W2355143312C71924100 @default.
- W2355143312 hasConceptScore W2355143312C7836513 @default.
- W2355143312 hasConceptScore W2355143312C86803240 @default.
- W2355143312 hasConceptScore W2355143312C95444343 @default.
- W2355143312 hasIssue "3" @default.
- W2355143312 hasLocation W23551433121 @default.
- W2355143312 hasOpenAccess W2355143312 @default.
- W2355143312 hasPrimaryLocation W23551433121 @default.
- W2355143312 hasRelatedWork W2136115206 @default.
- W2355143312 hasRelatedWork W2347271419 @default.
- W2355143312 hasRelatedWork W2349008764 @default.
- W2355143312 hasRelatedWork W2377852884 @default.
- W2355143312 hasRelatedWork W2378500573 @default.
- W2355143312 hasRelatedWork W2379669549 @default.
- W2355143312 hasRelatedWork W2382901031 @default.
- W2355143312 hasRelatedWork W2383262704 @default.
- W2355143312 hasRelatedWork W2384732060 @default.
- W2355143312 hasRelatedWork W3146033563 @default.
- W2355143312 hasVolume "28" @default.
- W2355143312 isParatext "false" @default.
- W2355143312 isRetracted "false" @default.
- W2355143312 magId "2355143312" @default.
- W2355143312 workType "article" @default.