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- W2355143469 abstract "L-5-methyltetrahydrofolic acid is the most active form of folic acid and is also the only form of folic acid which is able to cross the blood-brain barrier,so it has a great application value.To construct a recombinant strains capable of producing L-5-methyltetrahydrofolic acid,the gene of 5,10-methylenetetrahydrofolate reductase(metF) was cloned into vector pET-22b,and then the recombinant vector pET-22b-metF was transformed into E.coli BL21(DE3).The recombinant protein was induced by lactose,and finally the amount of L-5-methyltetrahydrofolic acid was improved.After the recombinant strain was induced by lactose,the amount of L-5-methyltetrahydrofolic acid of recombinant strain was increased by 28%,and the yield of L-5-methyltetrahydrofolic acid was up to 66μg/g cell.These results found a new way for the bio-synthesis of L-5-methyltetrahydrofolic acid." @default.
- W2355143469 created "2016-06-24" @default.
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- W2355143469 date "2011-01-01" @default.
- W2355143469 modified "2023-09-24" @default.
- W2355143469 title "Construction of Recombinant Strains Capable of Producing L-5-Methyltetrahydrofolic Acid" @default.
- W2355143469 hasPublicationYear "2011" @default.
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