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- W2355145301 abstract "Objective :The objective of this study was to investigate the responses of osteoblast to bioactivation (by micro-arc oxidation, M) and wear(by plasma nitriding, N) ; Ti6Al4V alloy was used as substrate for all coating procedures. Method: Osteoblast were isolated from crinial of neogenesis rat and the cell culture on that two coating surface in vitro .To determine cell proliferation was used MTT assay and their morphology observed by scanning electron microscope after 3 days. Alkaline phosphatase acivity was measured at 5d by ALP-special assay . These clearly demonstrate and quantify effect for Ti6Al4V under controlled experimental conditions . Result :The proliferateion rate and ALP activity of osteoblasts is not significant difference(P0.05) on two coating and control. M group appeared to show the highest ALP activity and cell proliferation.Ti6Al4V the lowest activity as well as proliferation and a little lower than that of N group. SEM show that the cells with multilayer appeared to be elongated and flattened, besides some rounded cells and filopodia on the M surface . Conclusion :The micro-arc oxidation with well cyto biocompatiblility due to an effect that enhancing osteoblasts proliferation and the cell activity morphocytology. Otherwith , the M ?N surface with higher level in alkalin phasphatase expression and proliferation percentage than control. Therefore, the micro-arc oxidation and plasma nitriding coating are better bioactive materials than Ti6Al4V." @default.
- W2355145301 created "2016-06-24" @default.
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- W2355145301 date "2006-01-01" @default.
- W2355145301 modified "2023-09-26" @default.
- W2355145301 title "Differential biological responses of osteoblasts on coating of Ti_6Al_4V by micro-arc oxidation and plasma nitriding" @default.
- W2355145301 hasPublicationYear "2006" @default.
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