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- W2355147851 abstract "AIM: To construct an adenoviral vector carrying rat FLIP(FLICE-inhibitory protein) gene and to observe its expression in rat pulmonary vascular endothelial cells(PVEC).METHODS: Recombined adenovirus Ad-FLIP was constructed by Ad-max system.The PVECs of rats were isolated,primarily cultured in vitro and infected with Ad-FLIP.The expression of FLIP was detected by Western blot and RT-PCR.RESULTS: FLIP gene was successfully cloned and incorporated into recombinant adenovirus Ad-FLIP.The expression of FLIP gene in the infected cells was detected by RT-PCR.The cells were infected with Ad-FLIP and FLIP protein was detected in the ingredients of cell disruption.CONCLUSION: The recombinant adenoviral vector carrying rat FLIP gene is successfully constructed and Ad-FLIP efficiently elevates the expression of FLIP gene and protein in cultured rat PVECs." @default.
- W2355147851 created "2016-06-24" @default.
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- W2355147851 date "2009-01-01" @default.
- W2355147851 modified "2023-09-27" @default.
- W2355147851 title "Construction of adenoviral expression vector carrying FLIP gene and its expression in rat pulmonary vascular endothelial cells" @default.
- W2355147851 hasPublicationYear "2009" @default.
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