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- W2355149721 abstract "Objective To optimize the procedure for purification and lysis of influenza virus vaccine.Methods Compare the purification efficacies by gel filtration chromatography and by sucrose density gradient centrifutgation,as well as the lysis efficacies with Triton X-100 and with sodium deoxycholate under various conditions.Results The purity of split influenza vaccine after purification by gel filtration chromatography was slightly higher than that by sucrose density gradient ultracentrifugation,while the recovery of vaccine by the latter was higher than that by the former.Triton X-100 showed good lysis efficacy as compared with sodium deoxycholate.After lysis with Triton X-100 at a final concentration of 0.5% or 0.8% for 3 or 4 h,more than 90% of influenza vaccine was lyzed.After storage at 4℃ for 12 months,all the quality indexes of the purified vaccine met the relevant requirements.However,after storage at 4℃for 18 months or at 37℃ for 28 d ,the HA content of vaccine was still more than 30 μg/ml.Conclusion A practical and feasible procedure for purification and lysis of influenza vaccine was developed." @default.
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- W2355149721 date "2008-01-01" @default.
- W2355149721 modified "2023-09-25" @default.
- W2355149721 title "Optimization of Procedure for Purification and Lysis of Influenza Virus Vaccine" @default.
- W2355149721 hasPublicationYear "2008" @default.
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