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- W2355159571 abstract "Objective:To investigate cytotoxic activity of LAK cells stimulated by dendritic cells(DCs)pulsed with lung cancer cell lysate(Ag)against lung cancer cell line A549 in vitro.Methods:DCs derived from the PBMC of lung cancer patients were induced by combination of GM-CSF,IL-4,TNF-a and pulsed with tumour freeze-thaw antigen,which had been cultured with LAK cell at ratio 1∶20 for 2 d(Ag-DC-LAK),and then these cultured cells were used to kill the A549 cell line cells in vitro.Ag-LAK,DC-LAK,LAK served as control group.Results:The cultured Ag-DC expressed by CD1a,CD80,CD86,HLA-DR were significantly higher than that of simple DC group(P0.05).The killing rate of Ag-DC-LAK to the lung cancer cell line A549 was (71±5)%;the rates in the groups of Ag-DC-LAK were significantly higher than that of the control group(P0.05).Conclusion:Dendritic cells (DCs) pulsed with lung cancer cell lysate(Ag)can enhance cytotoxic activity of LAK cells to cancer cells in vitro." @default.
- W2355159571 created "2016-06-24" @default.
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- W2355159571 date "2009-01-01" @default.
- W2355159571 modified "2023-09-23" @default.
- W2355159571 title "Cytotoxic Activity of LAK Cells Induced by Dendritic Cells against Lung Cancer Cell Line A549 in Vitro" @default.
- W2355159571 hasPublicationYear "2009" @default.
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