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- W2355162768 abstract "The GP5 gene which was spanned or truncated with hydrophobic signal peptide(tGP5) was amplified with RT-PCR and cloned into the downstream of promoter(CMV) of the pcDNA3.1,an eukaryotic expressing vector.The constructed plasmids were transfected into HEK-293A cells with anion lipid transfection reagent.Subsequently,the expression of the mRNA of interest gene was analyzed and confirmed by RT-PCR,and the expression of target protein was confirmed by indirect immunofluorescent assay(IFA).Each mouse was immunized with 100 μg of naked plasmid by intradermal injection.All groups were immunized four times with interval of 15 days.The neutralizing antibody against PRRSV were induced,but the antibody of group immunized with tGP5 gene produced slowly and the titer was lower than that in the group immunized with GP5 gene.It showed that the signal peptide of the GP5 protein of PRRSV is associated with the neutralizing antibody level of DNA immunization of pertinent GP5 gene." @default.
- W2355162768 created "2016-06-24" @default.
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- W2355162768 date "2005-01-01" @default.
- W2355162768 modified "2023-09-23" @default.
- W2355162768 title "Effect of N Terminal Signal Peptide of GP5 Protein on Responses to GP5 DNA Immunization of Porcine Reproductive and Respiratory Syndrome Virus" @default.
- W2355162768 hasPublicationYear "2005" @default.
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