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- W2355171890 abstract "A single step,single-tube reverse transcriptase-polymerase chain reaction(RT-PCR) test was developed to detect the presence of bovine viral diarrhoea virus(BVDV) in somatic cells from bulk milk samples.The test was validated by examining 50 bulk milk samples from 28 herds with result of BVDV elimination programme.Results showed that a total of 9 groups which contained one persistently infected animal were positive on bulk milk samples.Another 41 herds were negative except one herd which was positive because there was one acute infection cow.So the test was proved to be highly specific and sensitivity,which the positive predictive value(PPV) and the negative predictive value(NPV) were 0.9(9/10) and 0.98(40/41) respectively.In addition,50 bulk milk samples was tested by ELISA-Ab as well,and all of them were positive.There was no difference in OD of antibody between the positive herds and negative herds.Even if one positive samples could be found from 1000 samples in the laboratory.In the field test,the maximum sensitivity was for 380 cows.In one word,the one-step RT-PCR which was used in our test showed highly specific and sensitivity.Compared to bulk milk antibody test,the RT-PCR method could be more useful if herds with PI animals.The test cost would sharply reduce if samples was tested by RT-PCR and ELISA-Ag,therefore it was worth to recommend." @default.
- W2355171890 created "2016-06-24" @default.
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- W2355171890 date "2011-01-01" @default.
- W2355171890 modified "2023-09-25" @default.
- W2355171890 title "Application of One-step RT-PCR Used to Detect Bulk Milk in BVDV Eradication Programme" @default.
- W2355171890 hasPublicationYear "2011" @default.
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