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- W2355178223 abstract "AIM: To establish a simple rapid and sensitive hybridization method detecting products of asymmetric PCR(asy PCR) for HBV DNA from serum.METHODS:The upstream primer of Asy PCR of HBV DNA was modified with biotin and it′s concentration is 50 times of that of the downstream primer in the PCR system, so that the most products of PCR were single strand and labeled with biotin. Then the products were fixed upon the streptavidin coated wells. HRP labeled probes were added into wells ; Hybridization was completed in a short time and the latest hybridization signal was detected by ELISA liked assay. RESULTS: the sensitivity of the method was 10 100 times higher than that of the electrophoresis ; to the template purified with simple method, it`s adaptability and repeatability was better. CONCLUSION: The method is of potential use for routine examination of clinical specimens." @default.
- W2355178223 created "2016-06-24" @default.
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- W2355178223 date "1999-01-01" @default.
- W2355178223 modified "2023-09-25" @default.
- W2355178223 title "Hybridization colouring method of detecting the products of HBV DNA ASY PCR" @default.
- W2355178223 hasPublicationYear "1999" @default.
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