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- W2355178268 abstract "The PCR degenerate primers were designed from the conservative domain NBS-LRR (nucleotide binding siteand leucine-rich repeat) of most resistant disease genes in plants. The DNA fragments of 513 bp were amplified by genomicDNA and cDNA PCR of cabbage resistant material 84075, then cloned into multi-clone site of pUCm-T, transformed intoEscherichia coli DH5α, and sequenced. Four resistant gene analogs which contain NBS-LRR domain were obtained,named as Borl, Bor2, Bor3, Bor4. Their amino acid sequences have different homology scores compared with that ofresistance gene or resistance gene analog from other plants in Gene Bank. Using Bor1 as probe, Southern blot with genomicDNA of 84075 and RFLP analysis show Bor1 may be exist multi-copy." @default.
- W2355178268 created "2016-06-24" @default.
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- W2355178268 date "2004-01-01" @default.
- W2355178268 modified "2023-09-25" @default.
- W2355178268 title "Isolation of Resistant Gene Analog of NBS-LRR from Cabbage" @default.
- W2355178268 hasPublicationYear "2004" @default.
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