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- W2355228001 abstract "The transgenic mice carrying sFat-1 gene were produced using the sperm-mediated gene transfer method. The sperm cells were co-cultured with DNA fragments of sFat-1 gene, and then fertilization in vitro was performed and the embryos were transplanted into the uteri of pseudo-pregnant mice. Two out of sixteen offspring were found to be transgenic by PCR characterization, and further Western blotting analyses confirmed the two mice had integrated the sFat-1 gene into their genomes and the transgenic ratio was 12.5%. This study provided a useful animal model for future research on the biological function of sFat-1 gene." @default.
- W2355228001 created "2016-06-24" @default.
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- W2355228001 date "2008-01-01" @default.
- W2355228001 modified "2023-10-11" @default.
- W2355228001 title "Generation of sFat-1 Transgenic Mice by Sperm-mediated Gene Transfer" @default.
- W2355228001 hasPublicationYear "2008" @default.
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