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- W2355271463 abstract "To extract,isolate and identify the main protein allergens from wheat(Triticum aestivum)and buckwheat(Fagopyrum esculentum),the total protein from wheat and buckwheat were extract by PBS,the allergens were identified with 10 allergic patients' serum by Western-Blotting after SDS-PAGE.To partially purify the allergens,Ion exchange chromatography technique was employed.Finally immune activeness of the partially purify allergens was identified by means of Western-Blotting.In the crude extract from wheat,there are eighteen protein bands visible on the SDS-PAGE gel,and the most abundant 12 bands are 55 kD,42 kD,39 kD,36 kD,29 kD,27 kD,23 kD,21 kD,18 kD,15 kD,12 kD,9 kD proteins,respectively.Similarly,in the crude extract from Buckwheat,there are seventeen protein bands visible on the SDS-PAGE gel,and the most abundant 8 bands are 55 kD,44 kD,36 kD,32 kD,22 kD,16 kD,13 kD,10 kD proteins,respectively.The allergens from wheat with molecular mass of 36 kD,32 kD,29 kD,26 kD,23 kD,18 kD,15 kD,12 kD,9 kD were identified by Western-Blotting,and the primary allergen is 23 kD protein.The allergens from Buckwheat with molecular mass are 55 kD,36 kD,32 kD,29 kD,22 kD were identified by Western-Blotting.The primary allergen is 22 kD protein.The content of buckwheat allergens was raised from 10.73% to 21.07% after DE-52 anion exchange chromatography.Allergens from Triticum aestivum and Fagopyrum esculentum were isolated and identified.The result will provide a basis for the diagnosis and treatment of allergy to the allergens from Triticum aestivum and Fagopyrum esculentum." @default.
- W2355271463 created "2016-06-24" @default.
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- W2355271463 date "2008-01-01" @default.
- W2355271463 modified "2023-09-23" @default.
- W2355271463 title "Extraction,Isolation and Immunological Identification of Allergens from Triticum Aestivum and Fagopyrum Esculentum" @default.
- W2355271463 hasPublicationYear "2008" @default.
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