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• W2355283959 abstract "Most type II polyhydroxyalkanoate (PHA) synthases (PhaC) were found in Pseudomonas strains, and their genes are colocalized with PHA depolymerase (PhaZ) genes. Recently, two similar PHA biosynthesis gene clusters were cloned and analyzed from Pseudomonas nitroreducens and Burkholderia caryophylli in our lab. The deduced amino acid sequences of the three genes (phaC1, phaZ and phaC2) in the clusters were compared with those from six other Pseudomonas strains (Pseudomonas sp. 613, P. aeruginosa, P. aureofaciens, P. oleovorans, P. putida and P. resinovorans) containing the homologous pha clusters. The results showed that the three proteins PhaC1, PhaZ and PhaC2 shared high similarity. The pha clusters were conserved in the eight strains. The phylogenetic trees for PhaC1, PhaZ and PhaC2 of the eight strains were compared and same topology as that of the whole pha clusters (phaC1phaZphaC2 DNA sequence) from the eight strains was found. This result implied that the pha cluster in Pseudomonas spp. may arise from same ancestor. An ancient duplication event of PHA synthase genes to form the pha cluster may happen earlier than Pseudomonas strains. A hypothetical evolution history was proposed based on the analysis." @default.
• W2355283959 created "2016-06-24" @default.
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• W2355283959 date "2002-01-01" @default.
• W2355283959 modified "2023-09-25" @default.
• W2355283959 title "Cloning and Analysis of Genes Encoding Polyhydroxyalkanoate Synthases" @default.
• W2355283959 hasPublicationYear "2002" @default.
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