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- W2355290412 abstract "Duck Tembusu virus(DTMUV) infection leads to decrease dramatically in egg production of laying duck.However,no vaccine against DTMUV is available at present.To investigate the immunogenicity of DNA vaccine against DTMUV,the E gene of DTMUV was cloned into eukaryotic expression vector pCAGGS to construct pCAGGS-E and the expression of E protein was detected in pCAGGS-E transfected 293T cells by indirect immunofluorescence assay and western blot.Furthermore,the immunogenicity was evaluated in BALB/c mice with the liposome capsuled pCAGGS-E via intravenous injection.The specific antibodies were detected by indirect ELISA,which showed the antibody titers increased gradually after pCAGGS-E multiple inoculations.In conclusion,the pCAGGS-E was able to induced specific immune responses in mice." @default.
- W2355290412 created "2016-06-24" @default.
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- W2355290412 date "2012-01-01" @default.
- W2355290412 modified "2023-09-23" @default.
- W2355290412 title "Construction and immunogenicity of DNA vaccine encoding E gene of duck Tembusu virus" @default.
- W2355290412 hasPublicationYear "2012" @default.
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