Matches in SemOpenAlex for { <https://semopenalex.org/work/W235529788> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W235529788 endingPage "555" @default.
- W235529788 startingPage "551" @default.
- W235529788 abstract "A long-standing question in the biology of the intracellular bacterium, Chlamydia, has been the structure of the promoter recognized by its RNA polymerase. The ‘RNA polymerase sigma subunit paradox’ refers to the difficulty reconciling the conservation between the RNA polymerases of Chlamydia and Escherichia coli, especially at the level of the promoter-recognition sigma subunit, with the general lack of homology between chlamydial promoters and the E.coli σ70 consensus promoter. While the –10 promoter element appears to be conserved between Chlamydia and E.coli, the structure of the chlamydial –35 promoter element has not been defined. We have investigated the structure of the –35 element of the Chlamydia trachomatis dnaK promoter by measuring the effects of single base pair substitutions on in vitro promoter activity. Most substitutions produced large decreases in promoter activity, which allowed us to define the optimal –35 sequence in the context of the dnaK promoter. We found that the optimal chlamydial –35 promoter sequence is identical to the E.coli σ70 consensus –35 promoter element (TTGACA). These results indicate that the optimal promoter specificities of the major form of chlamydial RNA polymerase and E.coli σ70 RNA polymerase are in fact highly conserved. A further implication of our results is that many chlamydial promoters have a suboptimal promoter structure. We hypothesize that these chlamydial promoters are intrinsically weak promoters that can be regulated during the chlamydial developmental cycle by additional transcription factors." @default.
- W235529788 created "2016-06-24" @default.
- W235529788 creator A5032375446 @default.
- W235529788 creator A5046159408 @default.
- W235529788 date "2003-01-15" @default.
- W235529788 modified "2023-09-27" @default.
- W235529788 title "Mutational analysis of the Chlamydia trachomatis dnaK promoter defines the optimal -35 promoter element - eScholarship" @default.
- W235529788 hasPublicationYear "2003" @default.
- W235529788 type Work @default.
- W235529788 sameAs 235529788 @default.
- W235529788 citedByCount "0" @default.
- W235529788 crossrefType "journal-article" @default.
- W235529788 hasAuthorship W235529788A5032375446 @default.
- W235529788 hasAuthorship W235529788A5046159408 @default.
- W235529788 hasConcept C101762097 @default.
- W235529788 hasConcept C104317684 @default.
- W235529788 hasConcept C138885662 @default.
- W235529788 hasConcept C150194340 @default.
- W235529788 hasConcept C153911025 @default.
- W235529788 hasConcept C159047783 @default.
- W235529788 hasConcept C179926584 @default.
- W235529788 hasConcept C2776449523 @default.
- W235529788 hasConcept C2777391075 @default.
- W235529788 hasConcept C41895202 @default.
- W235529788 hasConcept C47319516 @default.
- W235529788 hasConcept C54355233 @default.
- W235529788 hasConcept C67705224 @default.
- W235529788 hasConcept C82381507 @default.
- W235529788 hasConcept C86803240 @default.
- W235529788 hasConceptScore W235529788C101762097 @default.
- W235529788 hasConceptScore W235529788C104317684 @default.
- W235529788 hasConceptScore W235529788C138885662 @default.
- W235529788 hasConceptScore W235529788C150194340 @default.
- W235529788 hasConceptScore W235529788C153911025 @default.
- W235529788 hasConceptScore W235529788C159047783 @default.
- W235529788 hasConceptScore W235529788C179926584 @default.
- W235529788 hasConceptScore W235529788C2776449523 @default.
- W235529788 hasConceptScore W235529788C2777391075 @default.
- W235529788 hasConceptScore W235529788C41895202 @default.
- W235529788 hasConceptScore W235529788C47319516 @default.
- W235529788 hasConceptScore W235529788C54355233 @default.
- W235529788 hasConceptScore W235529788C67705224 @default.
- W235529788 hasConceptScore W235529788C82381507 @default.
- W235529788 hasConceptScore W235529788C86803240 @default.
- W235529788 hasIssue "2" @default.
- W235529788 hasLocation W2355297881 @default.
- W235529788 hasOpenAccess W235529788 @default.
- W235529788 hasPrimaryLocation W2355297881 @default.
- W235529788 hasRelatedWork W1537608474 @default.
- W235529788 hasRelatedWork W1560410275 @default.
- W235529788 hasRelatedWork W1581263435 @default.
- W235529788 hasRelatedWork W1971042288 @default.
- W235529788 hasRelatedWork W2005166450 @default.
- W235529788 hasRelatedWork W2017988909 @default.
- W235529788 hasRelatedWork W2019607686 @default.
- W235529788 hasRelatedWork W2021464014 @default.
- W235529788 hasRelatedWork W2050452947 @default.
- W235529788 hasRelatedWork W2055279150 @default.
- W235529788 hasRelatedWork W2055631418 @default.
- W235529788 hasRelatedWork W2057523541 @default.
- W235529788 hasRelatedWork W2069142566 @default.
- W235529788 hasRelatedWork W2083629339 @default.
- W235529788 hasRelatedWork W2110265766 @default.
- W235529788 hasRelatedWork W2111512612 @default.
- W235529788 hasRelatedWork W2118641555 @default.
- W235529788 hasRelatedWork W2149656970 @default.
- W235529788 hasRelatedWork W2158360717 @default.
- W235529788 hasRelatedWork W2497790735 @default.
- W235529788 hasVolume "31" @default.
- W235529788 isParatext "false" @default.
- W235529788 isRetracted "false" @default.
- W235529788 magId "235529788" @default.
- W235529788 workType "article" @default.