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- W2355306969 abstract "Event-specific qualitative and quantitative polymerase chain reaction was established to quantitatively detect and identify genetically modified maize MON863.Transgenic event-specific primers and Taqman-MGB probes based on maize endogenous gene and the flanking sequence of MON863 were designed,and specificity of endogenous genes and exogenous gene was validated.The standard curves of endogenous reference genes and MON863 exogenous gene were established with the known transgenic percentage content samples of MON863.The transgenic percentage content of samples was tested using the curves.The results indicate this method was repeatable,special,sensitive and simple.The detection limit reached 0.001 ng/μL,namely 14 copies.This study can be widely used for qualitative and quantitative detection of MON863 genetically modified maize in import and export trade." @default.
- W2355306969 created "2016-06-24" @default.
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- W2355306969 date "2012-01-01" @default.
- W2355306969 modified "2023-09-27" @default.
- W2355306969 title "Quantitative detection of genetically modified maize MON863 by Taqman MGB real-time fluorescence PCR" @default.
- W2355306969 hasPublicationYear "2012" @default.
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