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- W2355320716 abstract "Objective To construct the adenovirus that express the extracellular domain of secretory human CD40LIg (sCD40 ligand Ig fusion protein). Methods The extracellular domain of human CD40L and IgGFc was amplified with PCR respectively and their PCR products were recovered and ligated with ligase to obtain sCD40LIg. The sCD40LIg was inserted into adenovirus expression vector pAdTrack-CMV to produce a recombinant vector pAdTrack-sCD40LIg and the recombinant vector was transduced into AdEasy-1-XL adenoviral vector system and acquired the recombinant sCD40LIg adenovirus. Results The recombinant adenovirus sCD40LIg was established by homologous recombinant and was identified by PCR, Western blotting and nucleotide sequencing. Conclusion The constructed recombinant sCD40LIg adenovirus vector provides a potent tool for gene therapy in the inducing of immune tolerance in the organ transplantation." @default.
- W2355320716 created "2016-06-24" @default.
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- W2355320716 date "2005-01-01" @default.
- W2355320716 modified "2023-09-25" @default.
- W2355320716 title "Construction, identification and expression of recombinant adenovirus expressing the gene of the extracellular domain of sCD40LIg in vitro" @default.
- W2355320716 hasPublicationYear "2005" @default.
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