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- W2355321520 abstract "Objective To establish the Real-time RT-PCR method for hand-foot-and-mouth disease (HFMD) and to provide a quick and accurate testing way for HMFD.Methods According to recommendation from the Guidelines of Hand-foot-and-mouth Disease Prevention (2009),Real-time RT-PCR method was used to detect human enterovirus,coxsackievirus A16,and enterovirus 71 in 82 HFMD cases clinically diagnosed from March to August,2009 in Zhuzhou.In addition,Real-time Quantitative RT-PCR was used to retest the cases.The results of the two tests were compared,and the accuracy and sensitivity of Real-time RT-PCT were analyzed.Results There were statistically significant differences in detection of human enterovirus,coxsackievirus A16,and enterovirus 71 in 82 HFMD cases between Real-time RT-PCT and RT-PCT (λ=4.09,P0.05).Real-time RT-PCR was more sensitive than RT-PCR.Conclusions This study has established a sensitive,easy and quick Real-time RT-PCR method for the detection of HFMD which provides a new way for quick test of HFMD." @default.
- W2355321520 created "2016-06-24" @default.
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- W2355321520 date "2010-01-01" @default.
- W2355321520 modified "2023-09-27" @default.
- W2355321520 title "Establishment and Application of Real-time RT-PCR Method for Detection of Enteroviruses of Hand,Foot and Mouth Disease" @default.
- W2355321520 hasPublicationYear "2010" @default.
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