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- W2355333831 abstract "Objective To establish a new,fast and efficient preparation method of myocardial infarction model.Methods Sprague-Dawley rats were anesthetized by using isoflurane gas,then myocardial infarction models were prepared by ligation of ramus descendens anterior arteriae coronaria sinistrae,after bluntly separation of intercostal muscle.Sham operation rat models were prepared by punctuation,not ligation,of ramus descendens anterior arteriae coronaria sinistrae.Small animal electrocardiograph,hemodynamic analyzer,2,3,5-triphenyltetrazolium chloride(TTC)staining and hematoxylin-eosin(HE)staining were used to identify myocardial infarction models.ResultsSurvival rates of myocardial infarction models and sham operation models were both 80.0%.Electrocardiograph analysis indicated that ST segment was raised apparently in myocardial infarction models on 2and 7days after operation.Hemodynamic detection indicated that diastolic blood pressure,systolic blood pressure,left ventricular systolic pressure and max ascending and descending rate of left ventricular pressure in myocardial infarction models were lower than sham operation models,but heart rate and heart ventricle end diastolic pressure were higher(P0.05).TTC staining showed that the color of infracted myocardium was white,which was obviously different with normal myocardium,HE staining showed that infracted cardiac muscle cells were swelling,disorderly arranged,with cavity or even break,and inflammatory cell infiltration could be found within intercellular spaces of fractured cardiac muscle cells.Conclusion Preparation method,constructed in this research,could be efficient and easily performed to establish myocardial infarction models." @default.
- W2355333831 created "2016-06-24" @default.
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- W2355333831 date "2015-01-01" @default.
- W2355333831 modified "2023-09-23" @default.
- W2355333831 title "Establishment and assessment of rat model of myocardial infarction" @default.
- W2355333831 hasPublicationYear "2015" @default.
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