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- W2355361615 abstract "OBJECTIVE:To establish a method for simultaneous determination of chlorogenic acid,geniposide and paeoniflorin in Compound huangbai capsules. METHODS:HPLC method was adopted. The determination was performed on ZORBAX Eclipse SB-C8(250 mm×4.6 mm,5 μm)column with acetonitrile-0.2‰ phosphoric acid as mobile phase(gradient elution)at the flow rate of 1.0 ml/min. The column temperature was at 30 ℃. The detection wavelengths were set at 327,240 and 232 nm for chlorogenic acid,geniposide and paeoniflorin,respectively. RESULTS:The linear ranges of chlorogenic acid,geniposide and paeoniflorin were 0.02-0.19 μg(r=0.999 7),0.09-0.84 μg(r=0.999 8)and 0.11-1.01 μg(r=0.999 9),respectively. RSDs of precision,stability and reproducibility tests were all lower than 3%. The average recoveries of the 3 components were 99.6%(RSD= 1.3%,n=6),101.0%(RSD=0.7%,n=6)and 100.2%(RSD=1.4%,n=6),respectively. CONCLUSIONS:The established method can be applied for the simultaneous determination of 3 components in Compound huangbai capsules." @default.
- W2355361615 created "2016-06-24" @default.
- W2355361615 creator A5022108591 @default.
- W2355361615 date "2014-01-01" @default.
- W2355361615 modified "2023-09-28" @default.
- W2355361615 title "Simultaneous Determination of 3 Components in Compound Huangbai Capsules by Multi-wavelength HPLC Method" @default.
- W2355361615 hasPublicationYear "2014" @default.
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