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- W2355362789 abstract "4-Diphosphocytidyl-2-C-methyl-D-erythritol(CDP-ME) synthetase gene was cloned from Mycobacterium tuberculosis H37Rv by PCR amplifi cation and expressed in E.coli BL21(DE3).The purifi ed protein was identifi ed as a homodimer with high specifi city for pyrimidine bases by HPLC and PAGE.Its maximal activity was found in the presence of cytidine triphosphate(CTP) and Mg2+.The Km for CTP and 2-C-methyl-D-erythritol-4-phosphate(MEP) is 92 and 43μmol/L.The Vmax is 7.8μmol·(min·mg)-1." @default.
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- W2355362789 date "2008-01-01" @default.
- W2355362789 modified "2023-09-25" @default.
- W2355362789 title "Purification and Characterization of a Recombinant 4-Diphosphocytidyl-2-C-methyl-D-erythritol Synthetase from Mycobacterium tuberculosis" @default.
- W2355362789 hasPublicationYear "2008" @default.
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