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- W2355371006 abstract "Objective: To establish the EA.hy926 cell line expressing granulocyte-macrophage colony stimulating factor(GM-CSF) and interleukin 4(IL-4) stably mediated by lentiviral system.Methods: The recombinant lentiviral plasmid carrying human GM-CSF and IL-4 was transfected into 293FT cells with lentiviral packaging plasmids.Then the infectious lentiviral was obtained,which infected the EA.hy926 cells.The expression of GM-CSF and IL-4 was assayed by qPCR and ELISA.Results: The GM-CSF and IL-4 expression at mRNA level of infected EA.hy926 cells were 24 and 9946 fold respectively for the FLSC not infected by the lentivirus,and then at the protein level were 145 and 23 fold.Conclusion: The infected EA.hy926 cells can stably express GM-CSF and IL-4." @default.
- W2355371006 created "2016-06-24" @default.
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- W2355371006 date "2011-01-01" @default.
- W2355371006 modified "2023-09-25" @default.
- W2355371006 title "Construction and Identification of Cell Line to Coexpress GM-CSF and IL-4 Mediated by Lentiviral System" @default.
- W2355371006 hasPublicationYear "2011" @default.
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