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- W2355373321 abstract "The ferritin gene in Exopalamon carinicauda was first cloned using RT-PCR and RACE and a 755bp cDNA was acquired.The result of sequencing indicated that the 5'-and 3'-untranslated regions(UTR) of this gene were 112bp and 146bp,respectively.It had an open reading frame of 497bp that encoded a 169 amino-acid polypeptid(Mr 19.1kDa),which had the isoelectric point(pI) of 5.46.The cDNA sequence was named Ecfer.Phylogenetic analysis using neighbor-joining on amino acid sequence from different species showed that Ecfer was in the same category as those in the crustaceans(homology was 64%—67%).In addition,the amino acid sequence of Ecfer shares 53%—57% similarity with the ferritin H in vertebrates.We used quantitative real-time PCR to estimate mRNA expression of Ecfer in organs or tissues and its induced expression in haemolymph,hepatopancreas of E.carinicauda following WSSV infection.The results showed that Ecfer expression existed in all tested organs or tissues of E.carinicauda,including hepatopancreas,ovary,muscle,gills,and haemolymph.3h and 6h after WSSV injection,the levels of Ecfer mRNA in hepatopancreas and haemolymph clearly increased,and this increase changed with time.We then concluded that Ecfer may be involved in the immune response of resisting WSSV invasion in E.carinicauda." @default.
- W2355373321 created "2016-06-24" @default.
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- W2355373321 date "2012-01-01" @default.
- W2355373321 modified "2023-09-30" @default.
- W2355373321 title "CLONING AND EXPRESSION ANALYSIS OF FERRITIN GENE IN EXOPALAEMON CARINICAUDA" @default.
- W2355373321 hasPublicationYear "2012" @default.
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