FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2355384022> ?p ?o ?g. }

• W2355384022 endingPage "241" @default.
• W2355384022 startingPage "237" @default.
• W2355384022 abstract "OBJECTIVE To investigate the effect of inhibition of nuclear factor kappa B on multiple organ injury following ruptured abdominal aortic aneurysm. METHODS Forty-five Wistar rats underwent catheterization to observe the intestinal microcirculation blood flow, and were randomly divided into 3 equal groups. Rats of the ruptured abdominal aortic aneurysm (RAAA) group underwent laparotomy and extraction of blood to cause hemorrhage shock for 1 h (hemorrhagic shock phase), by the end of this phase normal saline at the dose of 50 ml/kg was injected intravenously, after that the abdominal aorta and bilateral common iliac arteries were blocked with artery clamps for 45 min so as to cause lower torso ischemia, and then the extracted blood was reperfused. The lungs, small intestine were taken out to undergo histological examination, and examination of lung polymorphonuclear neutrophilic leukocyte (PMN) sequestration, lung wet tissues/dry (W/D) tissues ratio, and myeloperoxidase (MPO) activity. The rats of pyrrolidine dithiocarbamate (PDTC) group were perfused with PDTC, a specific inhibitor of nuclear factor kappa B (NF-kappaB), by the end of the hemorrhagic shock phase. And the rats of the sham operation group were perfused of normal saline. RT-PCR was used to detect the mRNA expression of NF-kappaB p65 and intercellular adhesion molecule (ICAM). Western blotting was used to detect the protein expression of NF-kappaB p65 and ICAM-1. Immunohistochemistry was used to detect the expression of NF-kappaB p65 and ICAM-1 in the lung and small intestine tissues. RESULTS Histological examination showed that severe damage could be found in the lung and small intestine of the RAAA group, and damages were significantly mild in the PDTC group. Lung PMN sequestration, W/D ratio, MPO activity were significantly increased in the RAAA group and these changes were relatively mild in the PDTC group (all P < 0.01). The intestinal microcirculation blood flow was 0.25 +/- 0.04 mlxmin(-1)xg(-1) in the RAAA group, significantly less than that of the sham operation group (0.71 +/- 0.11 mlxmin(-1)xg(-1), P < 0.01), and was 0.64 +/- 0.06 mlxmin(-1)xg(-1) in the PDTC group, significantly higher than that of the RAAA group (P < 0.01). The mRNA expression of NF-kappaB p65 in the lung of the RAAA group was 0.68 +/- 0.22, significantly higher than that of the sham operation group (0.11 +/- 0.02, P < 0.01) and that of the PDTC group (0.23 +/- 0.07, P < 0.01). The mRNA expression of NF-kappaB p65 in the intestine of the RAAA group was 0.48 +/- 0.10, significantly higher than that of the sham operation group (< 0.20 +/- 0.05, P < 0.01) and that of the PDTC group (0.27 +/- 0.06, P < 0.01). The mRNA expression of ICAM-1 in the lung of the RAAA group was 0.92 +/- 0.31, significantly higher than that of the sham operation group (0.07 +/- 0.02, P < 0.01) and that of the PDTC group (0.21 +/- 0.04, P < 0.01). The mRNA expression of ICAM-1 in the intestine of the RAAA group was 0.74 +/- 0.15, significantly higher than that of the sham operation group (0.14 +/- 0.05, P < 0.01) and that of the PDTC group (0.25 +/- 0.08, P < 0.01). The protein expression of NF-kappaB p65 in the lung of the RAAA group was 1.04 +/- 0.26, significantly higher than that of the PDTC group (0.52 +/- 0.13, P < 0.01). The protein expression of NF-kappaB p65 in the intestine of the RAAA group was 1.20 +/- 0.30, significantly higher than that of the PDTC group (0.64 +/- 0.21, P < 0.01). The protein expression of ICAM-1 in the lung of the RAAA group was 0.40 +/- 0.12, significantly higher than that of the PDTC group (0.18 +/- 0.06, P < 0.01). The protein expression of ICAM-1 in the intestine of the RAAA group was 0.46 +/- 0.15, significantly higher than that of the PDTC group (0.22 +/- 0.05, P < 0.01). Immunohistochemistry showed that NF-kappaB p65 and ICAM-1 positive cells were widely distributed in the lungs and intestine of the RAAA group and were rarely distributed in the sham operation group. CONCLUSION PDTC attenuates the multi-organ injury by inhibiting the expression of NF-kappaB p65, thus reducing the mRNA and protein expression of its downstream gene ICAM-1 gene." @default.
• W2355384022 created "2016-06-24" @default.
• W2355384022 creator A5001505881 @default.
• W2355384022 creator A5035646601 @default.
• W2355384022 creator A5039837606 @default.
• W2355384022 creator A5046590824 @default.
• W2355384022 creator A5062291083 @default.
• W2355384022 creator A5080492819 @default.
• W2355384022 creator A5091667643 @default.
• W2355384022 date "2006-01-24" @default.
• W2355384022 modified "2023-09-23" @default.
• W2355384022 title "[Inhibition of nuclear factor kappa B attenuates multiple organ injury following ruptured abdominal aortic aneurysm: an experiment with rats]." @default.
• W2355384022 doi "https://doi.org/10.3760/j:issn:0376-2491.2006.04.006" @default.
• W2355384022 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16677502" @default.
• W2355384022 hasPublicationYear "2006" @default.
• W2355384022 type Work @default.
• W2355384022 sameAs 2355384022 @default.
• W2355384022 citedByCount "0" @default.
• W2355384022 crossrefType "journal-article" @default.
• W2355384022 hasAuthorship W2355384022A5001505881 @default.
• W2355384022 hasAuthorship W2355384022A5035646601 @default.
• W2355384022 hasAuthorship W2355384022A5039837606 @default.
• W2355384022 hasAuthorship W2355384022A5046590824 @default.
• W2355384022 hasAuthorship W2355384022A5062291083 @default.
• W2355384022 hasAuthorship W2355384022A5080492819 @default.
• W2355384022 hasAuthorship W2355384022A5091667643 @default.
• W2355384022 hasConcept C126322002 @default.
• W2355384022 hasConcept C141071460 @default.
• W2355384022 hasConcept C142724271 @default.
• W2355384022 hasConcept C2776098176 @default.
• W2355384022 hasConcept C2777397205 @default.
• W2355384022 hasConcept C2777714996 @default.
• W2355384022 hasConcept C2779993416 @default.
• W2355384022 hasConcept C71924100 @default.
• W2355384022 hasConceptScore W2355384022C126322002 @default.
• W2355384022 hasConceptScore W2355384022C141071460 @default.
• W2355384022 hasConceptScore W2355384022C142724271 @default.
• W2355384022 hasConceptScore W2355384022C2776098176 @default.
• W2355384022 hasConceptScore W2355384022C2777397205 @default.
• W2355384022 hasConceptScore W2355384022C2777714996 @default.
• W2355384022 hasConceptScore W2355384022C2779993416 @default.
• W2355384022 hasConceptScore W2355384022C71924100 @default.
• W2355384022 hasIssue "4" @default.
• W2355384022 hasLocation W23553840221 @default.
• W2355384022 hasLocation W23553840222 @default.
• W2355384022 hasOpenAccess W2355384022 @default.
• W2355384022 hasPrimaryLocation W23553840221 @default.
• W2355384022 hasRelatedWork W1475054614 @default.
• W2355384022 hasRelatedWork W2360348496 @default.
• W2355384022 hasRelatedWork W2360766434 @default.
• W2355384022 hasRelatedWork W2364590151 @default.
• W2355384022 hasRelatedWork W2365198660 @default.
• W2355384022 hasRelatedWork W2365774574 @default.
• W2355384022 hasRelatedWork W2374395716 @default.
• W2355384022 hasRelatedWork W2378553578 @default.
• W2355384022 hasRelatedWork W2379973516 @default.
• W2355384022 hasRelatedWork W2382302730 @default.
• W2355384022 hasRelatedWork W2385360925 @default.
• W2355384022 hasRelatedWork W2387335936 @default.
• W2355384022 hasRelatedWork W2387752379 @default.
• W2355384022 hasRelatedWork W2388752123 @default.
• W2355384022 hasRelatedWork W2464423946 @default.
• W2355384022 hasRelatedWork W3028850062 @default.
• W2355384022 hasRelatedWork W3030255696 @default.
• W2355384022 hasRelatedWork W3030827432 @default.
• W2355384022 hasRelatedWork W3031822672 @default.
• W2355384022 hasRelatedWork W3032359240 @default.
• W2355384022 hasVolume "86" @default.
• W2355384022 isParatext "false" @default.
• W2355384022 isRetracted "false" @default.
• W2355384022 magId "2355384022" @default.
• W2355384022 workType "article" @default.