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- W2355417098 abstract "Chinese olive test-tube plantlets germinated from mature embryo were used as the experiment materials. Cloning and analysis of PPO, the transcriptions of PPO at different preservation stages in in vitro Chinese olive plantlets were studied, and the activity of PPO enzyme was also determined. The results indicated that 1 934 bp full-length cDNA sequence of PPO gene was obtained. It was registered in GenBank, and the accession number was JQ319005. The cDNA contained a 1 818 bp open reading frame(ORF), encoding 606 amino acids. Besides, a partial sequence of PPO was obtained(PPO1, JF811036). The predict of the protein showed PPO2 was alkaline, hydrophilic and non-secretory. PPO2 protein contained three typical conserved domains and might localize in mitochondrial matrix space. Serine predominance in phosphorylation, secondary structure was mainly as irregular curl. Three-dimensional structure prediction showed that Chinese olive in vitro plantlets PPO2 protein was surrounded with α-helice and irregular curl. Phylogenetic tree analysis of PPO2 showed there was a close relationship with Populus euphratica, but with a long distance with Vitis vinifera and Nelumbo nucifera. The analysis of qPCR showed preservation for 1 month, the plantlets with more young leaves, and the PPO2 gene expression was at the highest level. PPO activity of Canarium album plantlets changed slightly after preservation for 1~5 months; after preservation for 6~8 months, the PPO activity increased sharply. This research suggested PPO gene might be associated with the initiation of differentiation of tender tissue and organ, and PPO likely played an important role in the process of plantlet growth and development in Canarium album." @default.
- W2355417098 created "2016-06-24" @default.
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- W2355417098 date "2014-01-01" @default.
- W2355417098 modified "2023-09-26" @default.
- W2355417098 title "Cloning and Expression of Polyphenol Oxidase(PPO) Gene During Different Process of Preservation in in vitro Chinese Olive(Canarium album)" @default.
- W2355417098 hasPublicationYear "2014" @default.
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