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- W2355419202 abstract "AIM To develop a method of LC-MS/MS for determination of continine in human serum. METHODS After adding stable isotope chemical compound(continine-D3) as the internal standard,the analyte was achieved by direct serum protein precipitation and then XBridge~(TM) Phenyl(2.1 mm×150 mm, 5μm) was utilized for the analytical column,with 0.02%formic acid water solution-0.02%formic acid acetonitrile solution(83:17,V/V) as the mobile phase.After analysis by electrospray ionization source with positive ion Multiple Reaction Monitoring(MRM) scans,the outcomings of continine and continine-D3 were m/z 177.0→80.0 and m/z 180.5→80.2,respectively.RESULTS The linear calibration curve of continine showed excellent correlation in the concentration range of 10 - 2 000μg·L~(-1) with the lower limit of quantitation 10μg·L~(-1).The inter-and intra-run precision(RSD) were less than 5%and the average recovery rate of 77.9%to 88.6%.CONCLUSION The established LC-MS/MS method is shown to be sensitive, accurate,simple and suitable for the determination of continine in human serum." @default.
- W2355419202 created "2016-06-24" @default.
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- W2355419202 date "2010-01-01" @default.
- W2355419202 modified "2023-09-25" @default.
- W2355419202 title "LC-MS/MS method for determination of continine in human serum" @default.
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