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- W2355435562 abstract "Objective: This study for deleting of flagellin's central hypervariable domain,optimizating parameters of their protein's expression and purification was carried out,and it should contribute to gaining new and high effi-cient adjuvants actively.Methods: Have fished the genes of two flagellins FljB and FliC from genome of Salmonel-la typhimurium,we constructed the expression vectors for FljB and FliC and their relative mutants BNLC and CNLC,which variable regions were deleted selectively,then we induced the proteins expressin in E.coli,and puri-fied these proteins respectively.Results Conclusion: FljB and FliC expressed in dissoluble form in cells,and BNLC and CNLC expressed with inclusion bodies.Through optimizing the purification conditions,we setted up pu-rification methods of four proteins respectively,and getting apyrogenic proteins which purity exceed 95%.It estab-lished a foundation for studying the relationship of structure and adjuvant effiency of flagellin for the next step." @default.
- W2355435562 created "2016-06-24" @default.
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- W2355435562 date "2011-01-01" @default.
- W2355435562 modified "2023-09-25" @default.
- W2355435562 title "Prokaryotic Expression and Purification of Flagellins and Their Mutants as the Neotype Adjuvants" @default.
- W2355435562 hasPublicationYear "2011" @default.
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