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- W2355566476 abstract "Objective To isolate and identify the renal afferent arterial smooth muscle cells(RASMCs) in vitro. Methods Renal artery was isolated from male SD rats in sterile condition and was cannulated with ligatures. After the kidneys were perfused with an ice-cold physiological salt solution (PSS),the remaining cortical tissue was then pressed through mesh sieve and was digested by collagenase Ⅳ. To culture primary RASMCs,they were subsequently identified by a morphology method and by indirect immunofluorescence staining as a label for smooth muscle α-actin and myosin heavy chain. The cell viability was determined with Typan blue. Results RASMCs were identified by their morphology and immunohistochemical characters,with the cell viability of 96 %. Primary cultured cells could be subcultured after 7 days and successfully passaged for more than 10 times,without noticeable changes in morphology and growth characteristics. Conclusion The technique has the advantages of good reproducibility,high yield of pure RASMCs and quick growth of primary culture." @default.
- W2355566476 created "2016-06-24" @default.
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- W2355566476 date "2008-01-01" @default.
- W2355566476 modified "2023-09-23" @default.
- W2355566476 title "Primary Culture and Identification of Rat Renal Afferent Arterial Smooth Muscle Cells" @default.
- W2355566476 hasPublicationYear "2008" @default.
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