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- W2355568940 abstract "Semi_quantitative analysis is used for specific mRNA expression from minute quantity of cultured rabbit vascular endothelial cells.Total mRNA was abstracted from rabbit endothelial cells with QuickPrep D□R Micro mRNA Purification Kit.Using mRNA as a template,the relative content of Endothelin_1(ET_1)mRNA was analyzed by semi_quantitative reverse transcription and polymerase chain reaction(SqRT_PCR)method.Results showed that the undergraded mRNA was extracted from 10 5 cultured rabbit vascular endothelial cells,and its ratio of A 260 /A 280 was more than 1.8 .The quantity of ET_1 mRNA expression was determined with a method of SqRT_PCR.It is indicated that a rapid,simple,safe and reliable method of determining specific mRNA expression from minute quatity of cells is established." @default.
- W2355568940 created "2016-06-24" @default.
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- W2355568940 date "2000-01-01" @default.
- W2355568940 modified "2023-09-25" @default.
- W2355568940 title "Semi-Quantitative RT-PCR Analysis for Specific mRNA Expression from Minute Quantity of Rabbit Vascular Endothelial Cells" @default.
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