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- W2355574098 abstract "In order to analyze and predict the structural characteristics of glutathione S-transferase from Taenia asiatica by bioinformatics,a full-length cDNA sequence encoding glutathione S-transferase was identified from full-length cDNA plasmid libratory of Taenia asiatica by using the bioinformatics tools in bioinformatics webs site such as NCBI(http://www.ncbi.nlm.nih.gov/),ExPaSy(http://www.expasy.org/)and some else software packages of bioinformatics.The cDNA sequence was analyzed and the characteristics of its deduced protein including physical-chemical characteristics,epitopes,modification sites after translation,conserved domains,subcellular location,topological structure,the second and the three dimensional(3D)structure were predicted.The location of epitopes in predicted 3D structure of glutathione S-transferase was determined.It was demonstrated that the identity and positive rates between the amino acids sequence deduced by the cDNA sequence and that of glutathione S-transferase from Taenia solium are 94% and 97% respectively.This novel cDNA sequence contained 810bp with a putative open reading frame of 291 amino acids.The protein with stable physical-chemical characteristics had no transmembrane region and sequence for subcellular location.Three major linear epitopes of glutathione S-transferase was predicted to locate from 89 aa to 94 aa,27 aa to 32 aa and 119 aa to 124 aa.These three eiptopes were far away from each other on the surface of spatial structure of glutathione S-transferase,and two of which were consensus linear epitopes of other tape worms.In the present work,the full-length cDNA of Glutathione S-transferase was screened from the cDNA library of adult Taenia asiatica by bioinformatics method.It suggests that glutathione S-transferase of Taenia asiatica is predicted to be a cytosolic protein and a potential molecule for immunodiagnosis." @default.
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- W2355574098 date "2007-01-01" @default.
- W2355574098 modified "2023-09-24" @default.
- W2355574098 title "Cloning and bioinformatics analysis of cDNA encoding a novel glutathione S-transferase from Taenia asiatica" @default.
- W2355574098 hasPublicationYear "2007" @default.
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