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- W2355602477 abstract "T4 phage display is a newly developed system di splaying exogenous proteins or peptides on its surface. This process is initiate d by fusing a foreign gene to one coding small outer capsid protein (referred to SOC) or highly antigenic outer capsid protein (referred to HOC) of T4 phage sur face. In order to display the immunogen of classical swine fever virus(CSFV) ont o the surface of T4 phage a successful expression in fusion was obtained in this study by inserting SOC gene fused to 5′ end of a gene coding the most antigeni c polypeptide of CSFV E2 into T4 phage expression vector. The expressed fusion p rotein SOC-mE2 was visualized by SDS-PAGE with expected molecular size of 23.5 kd and the expression efficiency was estimated at 36.7%. The immunological test applying Western blot and ELISA showed that SOC-mE2 fusion protein could react with a panel of CSFV-specific polyclonal antisera against mE2 protein." @default.
- W2355602477 created "2016-06-24" @default.
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- W2355602477 date "2003-01-01" @default.
- W2355602477 modified "2023-09-25" @default.
- W2355602477 title "The Construction and Expression of CSFV E2-T4 Phage SOC Fused Gene" @default.
- W2355602477 hasPublicationYear "2003" @default.
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