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- W2355630216 abstract "Objective To prepare the ARL 1 recombinant protein and its specific antibody.Methods The ARL 1 cDNA was cloned into procaryotic expression vector PQE 30 and the recombinant ARL 1 was expressed in E.coli M15,The recombinant protein of ARL 1 was prepared;This protein was then used to immunize the healthy New Zealand rabbits to obtain the specific antibody against ARL 1.Results The recombinant protein of ARL 1 was expressed effectively in E.coli.After purified with Ni + NTA,the protein was used to immunize the healthy New Zealand rabbits to prepare the specific antibody against ARL 1.The titer of the antibody was 1∶20 000 according to the ring precipitation test and the double agar diffusion precipitation test.Conclusion The preparation of human ARL 1 recombinant protein and its specific antibody facilitates a possibility for further study of ARL 1 in the early diagnosis of liver cancer.This antibody can also be used to carry nadiate isotopas or drugs that resist tumour for taget killing of the liver cancer cells." @default.
- W2355630216 created "2016-06-24" @default.
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- W2355630216 date "2003-01-01" @default.
- W2355630216 modified "2023-09-25" @default.
- W2355630216 title "The Preparation and Identification of Human ARL-1 Recombinant Protein and Its Specific Antibody" @default.
- W2355630216 hasPublicationYear "2003" @default.
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