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- W2355642703 abstract "In order to break seed dormancy and explore the techniques of embryo culture in vitro in Swida wilsoniana,using mature embryos in S.wilsoniana as explants,the impact of sterilizing methods,low temperature,different hormone combinations and concentration on its development were researched through culture in vitro by means of single factor design.The results show that embryo is peeled and inoculated into medium under the aseptic condition after seeds without lignified exotesta were soaked in 0.1% HgCl2 solution for 10 minutes,it can reduce the contamination rate and ensure well survival rate.Refrigerating 3 days at 4 ℃ is of benefit to embryo germination.The highest germination rate of embryo,up to 83.33%,is obtained in MS medium supplemented with 0.4 mg/L KT,and aseptic seedling is healthy and strong and root system is developing well." @default.
- W2355642703 created "2016-06-24" @default.
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- W2355642703 date "2012-01-01" @default.
- W2355642703 modified "2023-09-27" @default.
- W2355642703 title "Techniques of mature embryo culture in vitro and rapid propagation in Swida wilsoniana" @default.
- W2355642703 hasPublicationYear "2012" @default.
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