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- W2355647534 abstract "Objective To develop a simple, sensitive, specific and quantitative method by competitive PCR for assay of hepatitis B virus amplicon produced. Methods Two capture probes, one for capturing wild fragments in PCR products and the other one for capturing mutant fragments, were designed. The capture probe can be coated to the surface of DNA binding well through the chemical binding between amino modified at 3′ end of probe and NOS group on wells. Heat denatured products were then added to wells and hybridization occurs between capture probe and biotin labeled strand of PCR amplicon. The hybridizing signal were detected with avidin alkaline phosphatase and its subtract. Results As low as 80ng/ml of interested fragment in PCR products which was not identifiable by agarose gel electrophoresis can be detected by this method. The wild and mutant fragment in competitive PCR products can be separately tested and then the amount of wild template before PCR can be calculated by using the formula introduced by Clementi et al. Conclusion With its simplicity, sensitivity, specificity and digitized results, the enzyme linked hybridization on microplate can be a method of choice for assay of competitive PCR products." @default.
- W2355647534 created "2016-06-24" @default.
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- W2355647534 date "2001-01-01" @default.
- W2355647534 modified "2023-09-26" @default.
- W2355647534 title "An enzyme linked hybridization on microplate for quantification of HBV gene amplicon after competitive PCR" @default.
- W2355647534 hasPublicationYear "2001" @default.
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