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- W2355647907 abstract "Objective:To observe the feasibility of transfection human nitric oxide synthase in 3T3 cells.Methods:The eukaryotic expression vector pcDNA3.0-iNOS was transfected into 3T3 cells.After screening with G418 and cloning,the cells were verified with RT-PCR and immunofluorescence staining. Results:the expression of iNOS mRNA and iNOS protein in 3T3 cells transfected with pcDNA3.0-iNOS was detected. Conclusion:the 3T3 cells transfected with pcDNA3.0-iNOS can be a useful tool to study the function of nitric oxide in vivo." @default.
- W2355647907 created "2016-06-24" @default.
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- W2355647907 date "2004-01-01" @default.
- W2355647907 modified "2023-09-23" @default.
- W2355647907 title "Expression and identification of human inducible nitric oxide synthase gene in 3T3 cells" @default.
- W2355647907 hasPublicationYear "2004" @default.
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