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- W2355664003 abstract "The study investigated the effect of epidermal growth factor on the development of zona-free mouse embryos in vitro.Zona of mouse pronuclear-stage embryos were removed by pronase,then the embryos were cultured in CZB media without serum,and the development rate of each stage of embryos were examined.As to the development rate of 2-cell and the recovery rate of blastocyst,the supplementation of CZB media with EGF at the concentration of 0.01 ng/mL was not significantly different from that using 0.1 ng/mL EGF,while the development rate of each stage,the recovery rate of blastocyst and the cell numbers of blastocyst was significantly higher than the other groups.The certain concentration of EGF can promote the development of each stage of zona-free mouse embryos in vitro,but the higher EGF can inhibited the development of zona-free mouse embryos." @default.
- W2355664003 created "2016-06-24" @default.
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- W2355664003 date "2009-01-01" @default.
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- W2355664003 title "Effect of epidermal growth factor on development of zona-free mouse embryos in vitro" @default.
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