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- W2355667062 abstract "Objective To investigate the effect of the serum after cardiopulmonary bypass(CPB) on human proximal tubular epithelial cells(HK-2) in vitro by improved ischemia reperfusion model.Methods HK-2 cell was used as target cell.The experiment was divided into 3 groups by culture medium,blank group(human serum-free medium),control group(medium containing normal human serum),test group(medium containing CPB serum,hypoxia and reoxygenation cultured).The apoptosis rate of HK-2 cell was detected by flow cytometry.Caspase-3 protein expression was detected by immunohistochemical technique.Result The apoptosis rate of HK-2 cell and Caspase-3 in test group were significantly higher than blank group and control group(P0.05),but there was no significant difference between blank group and control group.Conclusion CPB serum can induce HK-2 cell apoptosis,which mechanism may be related to the activation of cellular Caspase-3." @default.
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- W2355667062 date "2012-01-01" @default.
- W2355667062 modified "2023-09-25" @default.
- W2355667062 title "Injury of ischemia-reperfusion blood serum from patients under cardiopulmonary bypass on human proximal tubular epithelial cells" @default.
- W2355667062 hasPublicationYear "2012" @default.
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