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- W2355682941 abstract "OBJECTIVE To establish and validate a LC-MS/MS method for the determination of UP302 in rat liver microsome,and study the enzyme kinetics of UP302 metabolism in rat liver microsome.METHODS The LC-MS/MS method with mass transitions of m/z 301.1→135.2 for UP302 and 252.9→132.0 for internal standard daidzein was employed to determine UP302 incubated in rat liver microsome at negative electrospray ionization mode.Different incubation conditions,such as incubation time,substrate concentration and microsome concentration,were optimized.RESULTS The linear range of the calibration curve was 0.1-20 μmol·L-1 with good linear relationship.The intra-and inter-day accuracy was 86.42%-112.59%,and RSDs were less than 9.09%.The enzyme kinetic parameters were as follows:maximum reaction speed Vmax of 196.08 μmol·L-1·min-1·g-1,Km of 14.98 μmol·L-1,and CLint of 13.09 mL-1·min-1·g-1.CONCLUSION The established LC-MS/MS method was fast,simple,specific,sensitive and successfully applied to investigate the enzyme kinetics of UP302 metabolism in rat liver microsome." @default.
- W2355682941 created "2016-06-24" @default.
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- W2355682941 date "2011-01-01" @default.
- W2355682941 modified "2023-09-23" @default.
- W2355682941 title "Study on Metabolism Enzyme Kinetics of a Natural Novel Tyrosinase Inhibitor UP302 in Rat Liver Microsome by LC-MS/MS" @default.
- W2355682941 hasPublicationYear "2011" @default.
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