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- W2355695672 abstract "Objective Preparation of anti-human BPI23 monoclonal antibody with hybridoma technique and preliminary analysis of the application.Methods Theimmunized mouse spleen cells and mouse myeloma cells were fused by routine method;Indirect ELISA and Western-blot were used to screen the hybridoma cells;the positive clones were subcloned acquire hybridoma cells secreting anti-human BPI23 monoclonal antibody;the amplified hybridoma cells were injected into abdominal cavity of mouse and the ascites was collected;the antibodies in ascites were purified and titrated;the specificity of the antibody was analyzed by Western-blot;the normal human neutrophil and mononuclear cells were separated and purified for smear.Results Three hybridoma cell lines(1B4,9C12 and 2H11)secreting anti-human BPI23 monoclonal antibody were acquired.The anti-human BPI23 monoclonal antibodies secreted by hybridoma cells was kappa opioid IgM class,kappa opioid IgG1 subclass and kappa opioid IgG1 sub-class respectively;the titer was 1.28×105,1.28×105 and 4.1×106 respectively.Western-blot showed that the monoclonal antibody from ascites of tumor-bearing mice can recognize human BPI23 protein prepared in our lab and commercially available human BPI55 standards saled in the market specifically as well,but can't bind mouse BPI25 and human LBP;in immunohistochemistry.The 1B4,9C12 and 2H11 monoclonal antibody can recognize BPI of human neutrophil specifically.Conclusion The monoclonal antibody against human BPI23 was prepared successfully,which lay a foundation for later establishment of BPI detecting kit." @default.
- W2355695672 created "2016-06-24" @default.
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- W2355695672 date "2009-01-01" @default.
- W2355695672 modified "2023-09-25" @default.
- W2355695672 title "Preparation and identification of anti-human BPI_(23) monoclonal antibody" @default.
- W2355695672 hasPublicationYear "2009" @default.
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