Matches in SemOpenAlex for { <https://semopenalex.org/work/W2355696767> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2355696767 abstract "Objective To investiate effects of silencing expression of human telomerase reverse transcriptase(hTERT) gene by siRNA targeting to hTERT gene on the expressions of bcl-2,COX-2 genes of Capan-2 cells of human pancreatic cancer in vitro.Methods The media of transfection gene was Lipofectamine 2000.The expressions of hTERT mRNA,COX-2 mRNA and bcl-2 mRNA were tested by realtime PCR,and the control was GAPDH.The expressions of hTERT,COX-2 and bcl-2 proteins were tested by Western blotting,and the control was actin.Results Under the same experiment condition,the inhibited rate of expression of hTERT-mRNA by siRNA was 100%,and the expressions of COX-2,bcl-2 mRNA were inhibited remarkably by transfectation of hTERT-siRNA after 48 hours,and their inhibited rates were 100%,87.86% resprectively(P 0.001),but they recovered the same level as the control after 72 hours.Meanwhile,the expressions of COX-2,bcl-2 proteins were downregulated 50.06% and 82.77%,58.54% and 63.44% after 48 hours and 72 hours resprectively(P 0.01) .But no difference among the controls(P 0.05) .Conclusions Targing silence to hTERT gene by siRNA,it can inhibit the expressions of COX-2,bcl-2 genes of Capan-2 cells of human pancreatic cancer in vitro in some degree." @default.
- W2355696767 created "2016-06-24" @default.
- W2355696767 creator A5062582147 @default.
- W2355696767 date "2010-01-01" @default.
- W2355696767 modified "2023-09-23" @default.
- W2355696767 title "Effects of silencing expression of hTERT gene by siRNA on the expression of bcl-2 and COX-2 genes of human pancreatic cancer cells in vitro" @default.
- W2355696767 hasPublicationYear "2010" @default.
- W2355696767 type Work @default.
- W2355696767 sameAs 2355696767 @default.
- W2355696767 citedByCount "0" @default.
- W2355696767 crossrefType "journal-article" @default.
- W2355696767 hasAuthorship W2355696767A5062582147 @default.
- W2355696767 hasConcept C104317684 @default.
- W2355696767 hasConcept C105580179 @default.
- W2355696767 hasConcept C119056186 @default.
- W2355696767 hasConcept C125593758 @default.
- W2355696767 hasConcept C150194340 @default.
- W2355696767 hasConcept C153911025 @default.
- W2355696767 hasConcept C166703698 @default.
- W2355696767 hasConcept C185592680 @default.
- W2355696767 hasConcept C22615655 @default.
- W2355696767 hasConcept C40767141 @default.
- W2355696767 hasConcept C49923114 @default.
- W2355696767 hasConcept C502942594 @default.
- W2355696767 hasConcept C54009773 @default.
- W2355696767 hasConcept C54355233 @default.
- W2355696767 hasConcept C67705224 @default.
- W2355696767 hasConcept C86803240 @default.
- W2355696767 hasConcept C92087593 @default.
- W2355696767 hasConcept C94581717 @default.
- W2355696767 hasConceptScore W2355696767C104317684 @default.
- W2355696767 hasConceptScore W2355696767C105580179 @default.
- W2355696767 hasConceptScore W2355696767C119056186 @default.
- W2355696767 hasConceptScore W2355696767C125593758 @default.
- W2355696767 hasConceptScore W2355696767C150194340 @default.
- W2355696767 hasConceptScore W2355696767C153911025 @default.
- W2355696767 hasConceptScore W2355696767C166703698 @default.
- W2355696767 hasConceptScore W2355696767C185592680 @default.
- W2355696767 hasConceptScore W2355696767C22615655 @default.
- W2355696767 hasConceptScore W2355696767C40767141 @default.
- W2355696767 hasConceptScore W2355696767C49923114 @default.
- W2355696767 hasConceptScore W2355696767C502942594 @default.
- W2355696767 hasConceptScore W2355696767C54009773 @default.
- W2355696767 hasConceptScore W2355696767C54355233 @default.
- W2355696767 hasConceptScore W2355696767C67705224 @default.
- W2355696767 hasConceptScore W2355696767C86803240 @default.
- W2355696767 hasConceptScore W2355696767C92087593 @default.
- W2355696767 hasConceptScore W2355696767C94581717 @default.
- W2355696767 hasLocation W23556967671 @default.
- W2355696767 hasOpenAccess W2355696767 @default.
- W2355696767 hasPrimaryLocation W23556967671 @default.
- W2355696767 hasRelatedWork W118046654 @default.
- W2355696767 hasRelatedWork W1991511427 @default.
- W2355696767 hasRelatedWork W2000192089 @default.
- W2355696767 hasRelatedWork W2316390563 @default.
- W2355696767 hasRelatedWork W2360615591 @default.
- W2355696767 hasRelatedWork W2368179648 @default.
- W2355696767 hasRelatedWork W2395104695 @default.
- W2355696767 hasRelatedWork W2415566049 @default.
- W2355696767 hasRelatedWork W2418738339 @default.
- W2355696767 hasRelatedWork W2564056943 @default.
- W2355696767 hasRelatedWork W2578718647 @default.
- W2355696767 hasRelatedWork W2993875502 @default.
- W2355696767 hasRelatedWork W3028926320 @default.
- W2355696767 hasRelatedWork W3029522746 @default.
- W2355696767 hasRelatedWork W3030171709 @default.
- W2355696767 hasRelatedWork W3030773135 @default.
- W2355696767 hasRelatedWork W3030948936 @default.
- W2355696767 hasRelatedWork W3032847875 @default.
- W2355696767 hasRelatedWork W3142861461 @default.
- W2355696767 hasRelatedWork W3150537686 @default.
- W2355696767 isParatext "false" @default.
- W2355696767 isRetracted "false" @default.
- W2355696767 magId "2355696767" @default.
- W2355696767 workType "article" @default.