Matches in SemOpenAlex for { <https://semopenalex.org/work/W2355707553> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2355707553 endingPage "8" @default.
- W2355707553 startingPage "323" @default.
- W2355707553 abstract "To investigate the mechanisms of the drug resistance of gemcitabine resistance variant of the human lung adenocarcinoma cell line A549-Gem.Immunohistochemistry and RT-PCR were used to tested the expression of P-glycoprotein and transcription of mRNA of multidrug resistance gene and deoxycytidine kinase. Using a cDNA microarray compared the expression profiles between the resistant A549-Gem and the parent cell line A549.The A549-Gem shown slight positive expression of P-glycoprotein compared with positive control by immunohistochemistry, but A549 was negative for P-gp. RT-PCR amplified mRNA, using specific dCK and multidrug resistant gene 1 (mdr1) primers, demonstrated that A549-Gem express amplicon of mdr1 but no products of mdr1 was detected in A549. In the parent cell line, dCK mRNA amplication product could be detected, but did not found in resistant cells. About 18.8% of the total DNA elements had substantially altered level of expression in resistant A549-Gem compared with A549 by cDNA microarray. The biochemical functions of the different expressed genes are diverse and include oncogene and tumor suppressor gene, cell cycle regulator, heat shock protein, apoptotic and antiapoptotic factors, DNA transcription factors, DNA repair and recombination factors, signal transduction genes, protein translation genes, as well as a large number of metabolic genes. Several cluster genes overexpressed in resistant cell line, including ubiquitin-proteasome system, zinc finger protein, glutaredoxin and heat shock protein, may be related to the mechanisms of gemcitabine resistance.The mechanisms of resistance to gemcitabine are multifactorial, may include multidrug resistance and dCK deficiency. Differential expression genes monitored by cDNA microarray between A549-Gem and A549 may be related to the mechanisms of gemcitabine resistance in human lung cancer and potentially suggest the prevalence of expression of these genes in drug resistance. The use of cDNA microarray has provided a global view of the response of lung cancer cells to gemcitabine at the genomic level, it should be suitable for examining the development of drug resistance in cancer." @default.
- W2355707553 created "2016-06-24" @default.
- W2355707553 creator A5000122749 @default.
- W2355707553 creator A5006086141 @default.
- W2355707553 creator A5019846143 @default.
- W2355707553 creator A5029000628 @default.
- W2355707553 creator A5060544102 @default.
- W2355707553 creator A5065298628 @default.
- W2355707553 creator A5073931157 @default.
- W2355707553 creator A5079657615 @default.
- W2355707553 creator A5084795769 @default.
- W2355707553 date "2004-02-17" @default.
- W2355707553 modified "2023-09-25" @default.
- W2355707553 title "[Mechanisms of the drug resistance of a 2', 2-difluorodeoxycytide (gemcitabine)-resistant variant of the human lung adenocarcinoma cell line]." @default.
- W2355707553 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15059518" @default.
- W2355707553 hasPublicationYear "2004" @default.
- W2355707553 type Work @default.
- W2355707553 sameAs 2355707553 @default.
- W2355707553 citedByCount "0" @default.
- W2355707553 crossrefType "journal-article" @default.
- W2355707553 hasAuthorship W2355707553A5000122749 @default.
- W2355707553 hasAuthorship W2355707553A5006086141 @default.
- W2355707553 hasAuthorship W2355707553A5019846143 @default.
- W2355707553 hasAuthorship W2355707553A5029000628 @default.
- W2355707553 hasAuthorship W2355707553A5060544102 @default.
- W2355707553 hasAuthorship W2355707553A5065298628 @default.
- W2355707553 hasAuthorship W2355707553A5073931157 @default.
- W2355707553 hasAuthorship W2355707553A5079657615 @default.
- W2355707553 hasAuthorship W2355707553A5084795769 @default.
- W2355707553 hasConcept C104317684 @default.
- W2355707553 hasConcept C121608353 @default.
- W2355707553 hasConcept C150194340 @default.
- W2355707553 hasConcept C153911025 @default.
- W2355707553 hasConcept C2777844706 @default.
- W2355707553 hasConcept C2779268695 @default.
- W2355707553 hasConcept C2780258809 @default.
- W2355707553 hasConcept C29537977 @default.
- W2355707553 hasConcept C502942594 @default.
- W2355707553 hasConcept C54355233 @default.
- W2355707553 hasConcept C81729549 @default.
- W2355707553 hasConcept C81885089 @default.
- W2355707553 hasConcept C86803240 @default.
- W2355707553 hasConceptScore W2355707553C104317684 @default.
- W2355707553 hasConceptScore W2355707553C121608353 @default.
- W2355707553 hasConceptScore W2355707553C150194340 @default.
- W2355707553 hasConceptScore W2355707553C153911025 @default.
- W2355707553 hasConceptScore W2355707553C2777844706 @default.
- W2355707553 hasConceptScore W2355707553C2779268695 @default.
- W2355707553 hasConceptScore W2355707553C2780258809 @default.
- W2355707553 hasConceptScore W2355707553C29537977 @default.
- W2355707553 hasConceptScore W2355707553C502942594 @default.
- W2355707553 hasConceptScore W2355707553C54355233 @default.
- W2355707553 hasConceptScore W2355707553C81729549 @default.
- W2355707553 hasConceptScore W2355707553C81885089 @default.
- W2355707553 hasConceptScore W2355707553C86803240 @default.
- W2355707553 hasIssue "4" @default.
- W2355707553 hasLocation W23557075531 @default.
- W2355707553 hasOpenAccess W2355707553 @default.
- W2355707553 hasPrimaryLocation W23557075531 @default.
- W2355707553 hasRelatedWork W1898179592 @default.
- W2355707553 hasRelatedWork W2024930772 @default.
- W2355707553 hasRelatedWork W2029172019 @default.
- W2355707553 hasRelatedWork W2031076070 @default.
- W2355707553 hasRelatedWork W2057433089 @default.
- W2355707553 hasRelatedWork W2069943574 @default.
- W2355707553 hasRelatedWork W2089681493 @default.
- W2355707553 hasRelatedWork W2091358888 @default.
- W2355707553 hasRelatedWork W2100304286 @default.
- W2355707553 hasRelatedWork W2104435390 @default.
- W2355707553 hasRelatedWork W2107664894 @default.
- W2355707553 hasRelatedWork W2114714973 @default.
- W2355707553 hasRelatedWork W2121881270 @default.
- W2355707553 hasRelatedWork W2129191108 @default.
- W2355707553 hasRelatedWork W2132592067 @default.
- W2355707553 hasRelatedWork W2152427993 @default.
- W2355707553 hasRelatedWork W2411203715 @default.
- W2355707553 hasRelatedWork W2982139691 @default.
- W2355707553 hasRelatedWork W3165327243 @default.
- W2355707553 hasRelatedWork W1968401855 @default.
- W2355707553 hasVolume "84" @default.
- W2355707553 isParatext "false" @default.
- W2355707553 isRetracted "false" @default.
- W2355707553 magId "2355707553" @default.
- W2355707553 workType "article" @default.