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- W2355708545 abstract "Objective To study the method of foundation of human fetal adenohypophysial cell bank and the morphology and function of frozen-thawed(F-T)graft. Methods The human fetal adenohypophysial cells that were freezed when culturing for 5 days were thawed and recultured after 15 days. Compared to the simple culture group, they were monitored in the microscope and assayed by Typan blue and RIA. Results About 78. 2% F-T cells were survived and similar to primary cell in growing condition in the microscope. The GH secretion was the highest and the cell growing was best in the 5 ~ 7th day while the PRL and TSH secretion was low.Conclusion The way of this experiment is suitable to build the human fetal adenohypophysial cell bank for treating pituitary dwarfism. We should carry out clinic transplantation when the F-T cell have recultured for 5-7 days.The method is unfit for PRL secretion in freezing condition and for TSH secretion in culturing condition." @default.
- W2355708545 created "2016-06-24" @default.
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- W2355708545 date "2002-01-01" @default.
- W2355708545 modified "2023-09-23" @default.
- W2355708545 title "Foundation of human fetal adenohypophysial cell bank" @default.
- W2355708545 hasPublicationYear "2002" @default.
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